EnzyChrom™ Sucrose Assay Kit
Protocol
SDS
Application
- For quantitative determination of sucrose.
Key Features
- No interference by glucose.
- Use 20 μL samples. Linear detection range: 17 to 2000 μM sucrose.
Method
- OD565nm
Samples
- Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc
Species
- All
Size
- 100 tests
Detection Limit
- 17 μM
Shelf Life
- 6 months
More Details
- Sucrose (C12H22O11) is a disaccharide of glucose and fructose with a 1,2-glycosidic linkage. It is the most common food sweetener and the most important sugar in plants. In mammals, sucrose is readily digested in the stomach into glucose and fructose, which are rapidly absorbed into the bloodstream in the small intestine. Simple, direct and high-throughput assays for measuring sucrose concentrations find wide applications. BioAssay Systems improved assay uses invertase to digest sucrose into fructose and glucose. The resulting fructose is then quantified using our fructose assay reagent. The measured color intensity at 565nm is directly proportional to the sucrose concentration in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Zhu, M. et al. (2020). Identification and gene mapping of the starch accumulation and premature leaf senescence mutant ossac4 in rice. Journal of Integrative Agriculture, 19(9): 2150-2164. Assay: Sucrose in rice leaves.
Chen, X., Zhu, M., Gu, F., Liu, M., Zhang, Y., Xing, Y., & He, G. (2018). Identification and gene fine mapping of starch accumulation and early senescent leaf mutant esl10 in rice. Crop Science, 58(1), 204-217. Assay: Sucrose in microbe leaves.
Huang, J., Yan, M., Zhu, X., Zhang, T., Shen, W., Yu, P., & He, G. (2018). Gene mapping of starch accumulation and premature leaf senescence in the ossac3 mutant of rice. Euphytica, 214(10), 177. Assay: Sucrose in e. coli leaves.
Inukai, T. (2017). Differential Regulation of Starch-synthetic Gene Expression in Endosperm Between Indica and Japonica Rice Cultivars. Rice, 10(1), 7. Assay: Sucrose in rice karyopsis tissue.
Nakajima, Y., Maeda, K., Jin, Q., Takahashi-Ando, N., Kanamaru, K., Kobayashi, T., & Kimura, M. (2016). Oligosaccharides containing an alpha-( - 2)(glucosyl/xylosyl)-fructosyl linkage as inducer molecules of trichothecene biosynthesis for Fusarium graminearum. International journal of food microbiology, 238, 215-221. Assay: Sucrose in Fusarium graminearum cell lysate.
To find more recent publications, please click here.
Chen, X., Zhu, M., Gu, F., Liu, M., Zhang, Y., Xing, Y., & He, G. (2018). Identification and gene fine mapping of starch accumulation and early senescent leaf mutant esl10 in rice. Crop Science, 58(1), 204-217. Assay: Sucrose in microbe leaves.
Huang, J., Yan, M., Zhu, X., Zhang, T., Shen, W., Yu, P., & He, G. (2018). Gene mapping of starch accumulation and premature leaf senescence in the ossac3 mutant of rice. Euphytica, 214(10), 177. Assay: Sucrose in e. coli leaves.
Inukai, T. (2017). Differential Regulation of Starch-synthetic Gene Expression in Endosperm Between Indica and Japonica Rice Cultivars. Rice, 10(1), 7. Assay: Sucrose in rice karyopsis tissue.
Nakajima, Y., Maeda, K., Jin, Q., Takahashi-Ando, N., Kanamaru, K., Kobayashi, T., & Kimura, M. (2016). Oligosaccharides containing an alpha-( - 2)(glucosyl/xylosyl)-fructosyl linkage as inducer molecules of trichothecene biosynthesis for Fusarium graminearum. International journal of food microbiology, 238, 215-221. Assay: Sucrose in Fusarium graminearum cell lysate.
To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.