EnzyChrom™ Lactose Assay Kit
Protocol
SDS
Application
- For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.
Key Features
- Use as little as 20 μL samples. Linear detection range in 96-well plate: 17 to 2000 μM lactose for colorimetric assays and 6 to 100 μM for fluorimetric assays.
Method
- OD570nm, or FL530/585nm
Samples
- Biological (milk), food and agriculture etc
Species
- All
Size
- 100 tests
Detection Limit
- 6 μM
Shelf Life
- 6 months
More Details
- Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
I would like to know if I can use the optical density at 540nm to read my samples.
The signal at 540nm is only about 2/3 of the signal at 570 nm, but you still can use it. However, you will lose sensitivity.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Xue, H et al. (2020). Lactose-induced chronic diarrhea results from abnormal luminal microbial fermentation and disorder of ion transport in the colon. Frontiers in Physiology, 11, 877. Assay: Lactose in rat feces.
Snyder, N. A., Palmer, M. V., Reinhardt, T. A., & Cunningham, K. W. (2019). Milk biosynthesis requires the Golgi cation exchanger TMEM165. Journal of Biological Chemistry, 294(9), 3181-3191. Assay: Lactose in mouse skimmed milk.
Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44. Assay: Lactose in liposome.
Kwon DH et al (2012).Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. J Nutr Biochem 23(7):791-9. Assay: Lactose in rat milk.
To find more recent publications, please click here.
Snyder, N. A., Palmer, M. V., Reinhardt, T. A., & Cunningham, K. W. (2019). Milk biosynthesis requires the Golgi cation exchanger TMEM165. Journal of Biological Chemistry, 294(9), 3181-3191. Assay: Lactose in mouse skimmed milk.
Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44. Assay: Lactose in liposome.
Kwon DH et al (2012).Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. J Nutr Biochem 23(7):791-9. Assay: Lactose in rat milk.
To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.