QuantiFluo™ Protein Assay Kit
Protocol
SDS
Application
- For quantitative determination of total protein.
Key Features
- Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 μg/mL BSA.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- FL360/450nm
Samples
- Biological
Species
- All
Size
- 200 tests
Detection Limit
- 50 ng/mL
Shelf Life
- 6 months
More Details
- The protein or peptide is known as the "building blocks of life" and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable. BioAssay Systems QuantiFluo™ protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (λex/em = 360/450nm) is proportional to the protein concentration in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.