EnzyChrom™ Glucose Assay Kit III
Protocol
SDS
Application
- For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
Key Features
- Sensitive and accurate. Use as little as 20 μL samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
Method
- OD565nm
Samples
- Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc
Species
- All
Size
- 100 tests
Detection Limit
- 0.03 mM
Shelf Life
- 6 months
More Details
- Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery.
BioAssay Systems glucose assay kit III uses a single Working Reagent that combines the enzyme reaction and color reaction in one step. The color intensity of the reaction product at 565 nm is directly proportional to the glucose concentration in the sample.
What are the differences between the three EnzyChrom Glucose assay kits?
EBGL - best at detecting low levels of glucose (as low as 5 µM)EGL2 - best at measuring high levels of glucose using a low wavelength filter (340 nm)
EGL3 - best at measuring high levels of glucose using a high wavelength filter (565 nm)
Which of the three EnzyChrom Glucose assay kits can be used with urine, saliva, or serum samples?
EBGL - Not recommended for urine, saliva, or serum samplesEGL2 - Compatible with urine and serum samples, not recommended for saliva samples
EGL3 - Recommended for urine, saliva, and serum samples
*Sample blanks are to be used when testing urine samples with EGL2 or EGL3.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.