EnzyChrom™ Maltose Assay Kit

Protocol SDS

Application

• Quantitative determination of maltose in various biological samples such as serum, urine, food and beverages, etc.

Key Features

• Fast and sensitive. Use of 10 µL sample. Linear detection range 2 to 500 µM maltose in for colorimetric assays and 1 to 50 µM for flourimetric assays.
• Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• OD570nm, or FL530/585nm

Samples

• Serum, urine, food and beverages, etc.

Species

• All

Size

• 100 tests

Detection Limit

• 2 µM

Shelf Life

• 6 months

More Details

• MALTOSE(C₁₂H₂₂O₁₁) is a disaccharide, composed of two glucose units linked by an α bond. It is produced from the hydrolysis of glycogen or starch, serving as a source of energy for plants and animals. Maltose can be found in foods such as grains, and other processed products. BioAssay Systems maltose assay provides a simple, one step assay for measuring maltose. In this assay, maltose is converted to two glucoses, which are then oxidized to form a colored product. The color intensity of the product at 570 nm or fluorescence at λex/em = 530/585 nm is directly proportional to maltose concentration in the sample.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.