EnzyFluo™ Isocitrate Assay Kit

Protocol SDS

Application

• For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

Key Features

• Fast and sensitive. Linear detection range (20 μL sample): 0.6 to 500 μM for 10 min reaction.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/585 nm

Samples

• Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species

• All

Size

• 100 tests

Detection Limit

• 0.6 µM

Shelf Life

• 6 months

More Details

• ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems' isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.