EnzyChrom™ Glutathione Reductase Kit
Protocol
SDS
Application
- For quantitative determination of glutathione reductase activity.
Key Features
- Fast and sensitive. Linear detection range (20 μL sample): 0.4 to 50 U/L for 20 min reaction. Detection Limit of 0.4 U/L.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD412nm
Samples
- Plasma, serum, tissue and culture media etc
Species
- All
Size
- 100 tests
Detection Limit
- 0.4 U/L
Shelf Life
- 6 months
More Details
- GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. BioAssay Systems' non-radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman?s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
KHAN, ZAFAR JAVED, et al (2019). Indian Journal of Novel Drug Delivery. Indian Journal of Novel Drug Delivery 11.1: 30-39. Assay: Glutathione reductase in rat serum.
Bardell, DA et al (2015). Measures of Redox Balance in Horses Undergoing Corrective Surgery Involving Strangulating Lesions of the Small Intestine. Equine Vet J. 47(S48): 6. Assay: Glutathione reductase in horse plasma.
To find more recent publications, please click here.
Bardell, DA et al (2015). Measures of Redox Balance in Horses Undergoing Corrective Surgery Involving Strangulating Lesions of the Small Intestine. Equine Vet J. 47(S48): 6. Assay: Glutathione reductase in horse plasma.
To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.