EnzyChrom™ Citrate Assay Kit
Protocol
SDS
Application
- For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.
Key Features
- Fast and sensitive. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD570nm, or FL530/585nm
Samples
- Serum, plasma, agriculture etc
Species
- All
Size
- 100 tests
Detection Limit
- OD, FL: 4, 0.5 μM.
Shelf Life
- 6 months
More Details
- CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. BioAssay Systems' Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Pant, A. et al (2021). Viral growth factor-and STAT3 signaling-dependent elevation of the TCA cycle intermediate levels during vaccinia virus infection. PLoS Pathogens, 17(2). Assay: Citrate in human foreskin fibroblasts.
Fu, Xuekun, et al (2018). Runx2/Osterix and zinc uptake synergize to orchestrate osteogenic differentiation and citrate containing bone apatite formation. Advanced Science 5.4: 1700755. Assay: Citrate in mice cells.
Trivedi, Amit Kumar, et al. (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 184): 34-40. Assay: Citrate in bird plasma.
To find more recent publications, please click here.
Fu, Xuekun, et al (2018). Runx2/Osterix and zinc uptake synergize to orchestrate osteogenic differentiation and citrate containing bone apatite formation. Advanced Science 5.4: 1700755. Assay: Citrate in mice cells.
Trivedi, Amit Kumar, et al. (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 184): 34-40. Assay: Citrate in bird plasma.
To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
- Fast turnaround
- Quality data
- Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.